Internship and thesis proposals
Towards an integrated model of tip growth

Domaines
Biophysics
Soft matter
Physics of liquids
Physics of living systems

Type of internship
Expérimental
Description
Colonizing the world is one of the great challenges of living organisms. Animals move themselves, but other organisms, such as plants and fungi, rely mainly on growth.  Concentrating the elongation in the tip is a solution allowing organisms to quickly adapt growth to the properties of their environment. Therefore, tip growth is a convergent evolution observed for cells as distant in the phylogeny as fission yeast, fungal hyphae, plant pollen tube or plant root hair. Root hairs are cylindrical extensions which grow laterally from the root epidermis. While they keep a typical cell diameter of 10 µm, root hairs can reach ~1 mm in length, thus significantly increasing the surface area of roots, enhancing their ability to uptake essential resources for plant growth and development. In this context, we have designed original micro-mechanical techniques, combined with microfluidic devices, allowing us to measure, in vivo, many parameters of the growing root hair, as well as the nucleus movements, allowing us now to get an integrated quantitative description of tip growth. We propose an internship dedicated to such mechanical measurements after hyper-osmotic shocks in order to gain insight into growth regulation. The internship could also involve image analysis (PIV) to analyse the effect of a stiffening environment on nucleus dynamics. This internship could be followed by a PhD dedicated to defining an integrated model of tip growth.

Contact
Atef Asnacios
0678269051


Email
Laboratory : MSC - UMR7057
Team : Physique du vivant
Team Website
/ Thesis :    Funding :